- family: Ctenidae
- genus: Acantheis Thorell, 1891
- genus: Acanthoctenus Keyserling, 1877
- genus: Africactenus Hyatt, 1954
- genus: Amauropelma Raven, Stumkat & Gray, 2001
- genus: Anahita Karsch, 1879
- genus: Ancylometes Bertkau, 1880
- genus: Apolania Simon, 1898
- genus: Asthenoctenus Simon, 1897
- genus: Bengalla Gray & Thompson, 2001
- genus: Caloctenus Keyserling, 1877
- genus: Celaetycheus Simon, 1897
- genus: Centroctenus Mello-Leitao, 1929
- genus: Ctenopsis Schmidt, 1956
- genus: Ctenus Walckenaer, 1805
- genus: Cupiennius Simon, 1891
- genus: Diallomus Simon, 1897
- genus: Enoploctenus Simon, 1897
- genus: Gephyroctenus Mello-Leitao, 1936
- genus: Incasoctenus Mello-Leitao, 1942
- genus: Isoctenus Bertkau, 1880
- genus: Itatiaya Mello-Leitao, 1915
- genus: Janusia Gray, 1973
- genus: Leptoctenus L. Koch, 1878
- genus: Mahafalytenus Silva, 2007
- genus: Montescueia Carcavallo & Martínez, 1961
- genus: Nothroctenus Badcock, 1932
- genus: Paravulsor Mello-Leitao, 1922
- genus: Petaloctenus Jocqué & Steyn, 1997
- genus: Phoneutria Perty, 1833
- genus: Phymatoctenus Simon, 1897
- genus: Pseudoctenus Caporiacco, 1949
- genus: Thoriosa Simon, 1910
- genus: Trogloctenus Lessert, 1935
- genus: Trujillina Bryant, 1948
- genus: Tuticanus Simon, 1897
- genus: Viracucha Lehtinen, 1967
- genus: Viridasius Simon, 1889
- genus: Vulsor Simon, 1889
- genus: Wiedenmeyeria Schenkel, 1953