Sequencing

Sequencing

Set up in Eppendorf plate.

Recipe:

5uL cleaned PCR product
1uL Big Dye Extender Buffer
1uL 3.2 uM primer
1uL Big Dye
Seal with wax or heat seal.

Thermal profile:

Basic program
94 C :30
50 C 1:00
60 C 4:00
35 cycles
hold at 4 C